|
| Status |
Public on Jul 09, 2024 |
| Title |
Pre 7 |
| Sample type |
SRA |
| |
|
| Source name |
Blood
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Blood
cell type: PBMC
|
| Growth protocol |
Cultured C-225 cells were resuspended in sterile PBS and Matrigel in a 1:1 ratio. Subsequently, 2 million tumor cells were injected subcutaneously into the flank regions of each mouse.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
PBMCs were isolated from peripheral blood samples collected from C-225 tumor-bearing mice (n=7) on day 102 (pre-PBMC) and day 116 (post-PBMC) after the first tumor inoculation. Each mouse has 2 samples sequenced including pre- and post-PBMC, tumor rechallenge was on day 109. RBC was lysed and genomic DNA was purified.
ImmunoSEQ mmTCRB Service (Survey)
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Adaptive Biotechnologies’ ImmunoSEQ platform
|
| Data processing |
Processed data file from Immunoseq data analyzer for each sample is submitted
Assembly: mm10
Supplementary files format and content: Processed data files from Immunoseq data analyzer
|
| |
|
| Submission date |
Jul 02, 2024 |
| Last update date |
Jul 09, 2024 |
| Contact name |
Jing Hong Wang |
| E-mail(s) |
jhw51@pitt.edu
|
| Phone |
4128647728
|
| Organization name |
University of Pittsburgh
|
| Department |
Department of Medicine
|
| Lab |
Jing Hong Wang
|
| Street address |
5117 Centre Ave, Suite 2.35
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213-1862 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE271371 |
Differential tumor immune microenvironment coupled with tumor progression or tumor eradication in HPV-antigen expressing squamous cell carcinoma (SCC) models. |
|
