|
| Status |
Public on Apr 01, 2012 |
| Title |
Tumor 1841 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: oral cavity squamous cell carcinoma
|
| Treatment protocol |
Tumors were collected at the time of surgical resection and snap frozen in liquid nitrogen. Tumor and normal tissue were histologically confirmed. Frozen slides were microdissected to enrich tumor content to >70%.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using the Qiagen blood and tissue kit
|
| Label |
Cy5
|
| Label protocol |
Genomic DNA were fragmented and labelled Cy5 and Cy3 according to the standard Agilent protocol from 1-3ug total DNA.
|
| |
|
| Channel 2 |
| Source name |
Human genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Human Genomic DNA (Roche catalog. 11691112001)
|
| Treatment protocol |
Tumors were collected at the time of surgical resection and snap frozen in liquid nitrogen. Tumor and normal tissue were histologically confirmed. Frozen slides were microdissected to enrich tumor content to >70%.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using the Qiagen blood and tissue kit
|
| Label |
Cy3
|
| Label protocol |
Genomic DNA were fragmented and labelled Cy5 and Cy3 according to the standard Agilent protocol from 1-3ug total DNA.
|
| |
|
| |
| Hybridization protocol |
Labelled DNA-Cy5 hybridized together with matching labelled-Cy3 reference for 40 hrs at 65C on SurePrint G3 Human CGH Microarray Kit, 1x1M Array.
|
| Scan protocol |
Agilent Chips were washed and Scanned according to the standard Agilent protocol using the High Resolution Agilent Scanner.
|
| Data processing |
Feature Extraction Software v10.9.3 (Agilent)
|
| |
|
| Submission date |
Nov 28, 2011 |
| Last update date |
Apr 01, 2012 |
| Contact name |
Luc GT Morris |
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL8736 |
| Series (1) |
| GSE33983 |
Array comparative genomic hybridization in oral cavity (head and neck) squamous cell carcinoma |
|
