|
| Status |
Public on Apr 17, 2012 |
| Title |
Toh2-Male-Offspring1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Male offspring 1 gDNA
|
| Organism |
Danio rerio |
| Characteristics |
Sex: Male
strain: Toh
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Tail fin genomic DNA extracted using QIAGEN Dneasy Blood and Tissue kit according to manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Labeled according to NimbleGen Dual-Color Labelling Kit protocol.
|
| |
|
| Channel 2 |
| Source name |
Paternal gDNA
|
| Organism |
Danio rerio |
| Characteristics |
reference: Paternal gDNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Tail fin genomic DNA extracted using QIAGEN Dneasy Blood and Tissue kit according to manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Labeled according to NimbleGen Dual-Color Labelling Kit protocol.
|
| |
|
| |
| Hybridization protocol |
Standard NimbleGen protocol.
|
| Scan protocol |
Arrays were scanned with Axon GenePix 4000B scanner as per standard NimbleGen protocol (version 5.1) .
|
| Description |
Family Toh2 male offspring 1 / Paternal gDNA
|
| Data processing |
Arrays were processed using Nimblegen's standard protocol for Nimblescan 2.6 aCGH data extraction.
|
| |
|
| Submission date |
Dec 11, 2011 |
| Last update date |
Apr 18, 2012 |
| Contact name |
Woei Chang Liew |
| E-mail(s) |
wcliew@tll.org.sg
|
| Organization name |
Temasek Life Sciences Laboratory
|
| Lab |
Reproductive Genomics
|
| Street address |
1 Research Link, NUS
|
| City |
Singapore |
| ZIP/Postal code |
117604 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL13500 |
| Series (2) |
| GSE34337 |
Toh family 2 |
| GSE34338 |
Array comparative genomic hybridization to identify genomic differences between male and female zebrafish. |
|
