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Sample GSM848747 Query DataSets for GSM848747
Status Public on Dec 14, 2011
Title Day 70 CPD Control, Biological Rep1
Sample type RNA
Source name Day 70 CPD Control
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: Male
tissue: Whole Brain (without olfactory bulb)
treament: Matched Control
model: CPD
# samples pooled: 2
Treatment protocol Day 70 male mice were subjected to CO2 asphyxiation followed by cervical disolaction. Whole brain tissue was dissected in under five minutes and flash frozen liquid nitrogen.
Growth protocol C57BL/6J mice were prenatally exposed to ethanol either via Trimister 1,2, or 3, injections or continous preference drinking (Labelled T1, T2, T3, or CPD, respectively).
Extracted molecule total RNA
Extraction protocol TRIzol according to manufacturers protocol
Label Biotin
Label protocol One microgram of total RNA was labeled using the Flash Tag Biotin HSR kit (Genisphere)
Hybridization protocol Samples were then hybridized to Affymetrix miRNA 2.0 arrays for 16 hours at 45°C
Scan protocol Probe level (.CEL file) data was generated using Affymetrix Command Console v1.1.
Description miRNA expression data from Day 70 males that are matched controls for CPD mice
Data processing Probes were summarized to gene level data in Partek Genomics Suite v6.6 (Partek, St. Louis, MO, USA) using the RMA algorithm. Partek software was used to determine ANOVA p-values and fold changes.
Submission date Dec 13, 2011
Last update date Dec 14, 2011
Contact name Benjamin I Laufer
Organization name Genentech
Street address 1 DNA Way
City South San Francisco
State/province California
ZIP/Postal code 94080
Country USA
Platform ID GPL14613
Series (1)
GSE34413 Persistent Alterations in the imprinted miRNA and snoRNA populations in mouse models of fetal alcohol spectrum disorders (FASD)

Data table header descriptions
VALUE log2 RMA signal

Data table
1367452_st 3.62629
1367453_st 2.90242
1367454_st 3.91365
1367455_st 3.25739
1367456_st 3.0273
1367457_st 2.96282
1367458_st 2.80468
1367459_st 3.27755
1367460_st 2.75268
1367461_st 2.83367
1367462_st 2.74231
1367463_st 2.96282
1367464_st 4.05349
1367465_st 2.57568
1367466_st 2.74103
1367467_st 2.51899
1367468_st 5.13651
1367469_st 3.75793
1367470_st 2.96282
1367471_st 2.78272

Total number of rows: 20706

Table truncated, full table size 498 Kbytes.

Supplementary file Size Download File type/resource
GSM848747_M0019.CEL.gz 590.4 Kb (ftp)(http) CEL
Processed data included within Sample table

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