|
| Status |
Public on Sep 12, 2024 |
| Title |
JHW 2 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral Blood, MTB infected model treated by JHW, replicate 2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: PBMC
gender: female
treatment: MTB infected mouse treated by JHW
|
| Treatment protocol |
3 days after MTB infection, 50 mice were randomly divided into the following 5 groups and orally administered 5 times a week: TB model group (0.5mL distilled water/day per mouse), JHW treatment group (12mg/day per mouse), low-dose (2.4mg/day per mouse) of NBXH intermediate group, middle-dose (4.8mg/day per mouse) of NBXH intermediate group, and high-dose (9.6mg/day per mouse) of NBXH intermediate group.
|
| Growth protocol |
Sixty BALB/c 42-62-day-old female mice were purchased from Beijing Vitalriver Experimental Animal Technology Co., LTD., and put into the animal laboratory of the Eighth Medical Center of the PLA General Hospital for adaptive feeding.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Three weeks after the last immunization, three mice in each group mentioned above were euthanized, and then their blood was collected in heparin sodium anticoagulant tubes. According to the manufacturer's instructions, PBMCs were isolated with the Mouse PBMC Isolation Kit (Haoyang Biological Products Technology, Tianjin, China), and total RNA was extracted from PBMCs using TRIzol Reagent (Invitrogen, USA).
|
| Label |
Cy3
|
| Label protocol |
repare labeling reactionKit:Quick Amp Labeling Kit, One-Color (Agilent p/n 5190-0442)Procedure:1.
|
| |
|
| Hybridization protocol |
HybridizationKit and Instruments:Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242):10X Blocking Agent25X Fragmentation Buffer2x GEx Hybridization Buffer HI-RPMHybridization Chamber, stainless (Agilent p/n G2534A)Hybridization Chamber gasket slides (Agilent p/n G2534-60003)Hybridization oven (Agilent p/n G2545A)Hybridization oven rotator for Agilent Microarray Hybridization Chambers (Agilent p/n G2530-60029)Procedure:Add 500 μL of nuclease-free water to the vial containing lyophilized 10X Blocking Agent. Mix by gently vortexing.Equilibrate water bath to 60°C.For each microarray, add each of the components as indicated in the tables as below to a 1.5 mL nuclease-free microfuge tube:2.
|
| Scan protocol |
Instrument:Agilent Microarray Scanner (Agilent p/n G2565BA)Procedure:1.
|
| Description |
Gene expression of MTB infected mouse treated by JHW
|
| Data processing |
Extract data using Agilent Feature Extraction SoftwareSoftware:Agilent Feature ExtractionProcedure:1.
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| |
|
| Submission date |
Sep 11, 2024 |
| Last update date |
Sep 12, 2024 |
| Contact name |
Nan Wang |
| E-mail(s) |
liuk@cqu.edu.cn
|
| Organization name |
People's Liberation Army General Hospital
|
| Street address |
Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Senior Department of Tuberculosis, Eighth Medical Center of PLA General Hospital
|
| City |
beijing |
| ZIP/Postal code |
100091 |
| Country |
China |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE276937 |
Transcriptomics-based anti-tuberculous mechanism of traditional Chinese polyherbal preparation NiuBeiXiaoHe intermediates |
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