|
| Status |
Public on Sep 13, 2024 |
| Title |
158028 WT 24M H3K27me3 |
| Sample type |
SRA |
| |
|
| Source name |
brain
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain
cell type: neurons
genotype: WT
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Mice aged and samples collected at 3, 12, 24m, purified neurons extracted with FACS
Nugen Ovation Ultralow V2 library preparation - amplification for 17 cycles (H3K27ac), or 18 cycles (H3K4me3) 0347 V2 1-96/0344 V2 1-16.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequenced using HiSeq 2500 - mean read depth 13.9M H3K4me3, 16.2M H3K27ac.
Removed library tags.
Pseudoreplicates generated.
Mapped to MM10 using bowtie2.
bigwigs generated.
Assembly: MM10
Supplementary files format and content: Bigwigs
|
| |
|
| Submission date |
Sep 13, 2024 |
| Last update date |
Sep 13, 2024 |
| Contact name |
Phillippa Claire Taberlay |
| E-mail(s) |
Phillippa.Taberlay@utas.edu.au, Phillippa.Taberlay@gmail.com
|
| Organization name |
University of Tasmania
|
| Department |
College of Health and Medicine
|
| Street address |
17 Liverpool St
|
| City |
Hobart |
| State/province |
Tasmania |
| ZIP/Postal code |
7000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE190102 |
Ageing-Related Changes to H3K4me3, H3K27ac and H3K27me3 in Purified Mouse Neurons |
|
| Relations |
| BioSample |
SAMN43761774 |
| SRA |
SRX26079624 |
