|
| Status |
Public on Dec 28, 2011 |
| Title |
sphere stage zebrafish embryo_nanog-like-MO1_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
sphere stage zebrafish embryo_nanog-like-MO1
|
| Organism |
Danio rerio |
| Characteristics |
genotype: nanog-like-MO1
phenotype: gastrulation defect
|
| Growth protocol |
E3 fish water, 28°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by Trizol reagent (Invitrogen).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by Nimblegen using their standard protocol
|
| |
|
| Hybridization protocol |
Hybridization was performed by Nimblegen using their standard protocol
|
| Scan protocol |
Scanning was performed by Nimblegen using their standard protocol
|
| Description |
39658602_532
It is the first of second nanog-like-MO1-injected biological replicates used in this experiment, each from separate cultures.
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Dec 22, 2011 |
| Last update date |
Dec 28, 2011 |
| Contact name |
Cong Xu |
| E-mail(s) |
congxu@fas.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Street address |
1 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02134 |
| Country |
USA |
| |
|
| Platform ID |
GPL14375 |
| Series (2) |
| GSE34682 |
Expression analysis of Nanog-like-MO1 injected zebrafish embryos at the sphere stage |
| GSE34684 |
Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway |
|
