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Status
Public on Dec 28, 2011
Title
preT.ETP.Th#6
Sample type
RNA
Source name
thymus
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Filter/syringe plunger teasing, ACK lysis Lin+ magnetic depletion (Dynobeads), 2 rounds of sorting, 2d sort directly into trizol
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
preT.ETP.Th#6, Early T Lineage Progenitor
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Dec 27, 2011
Last update date
Jul 21, 2021
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE46349 Reanalyzed by
GSE180556
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
17.7246
10344622
257.7636
10344624
351.1993
10344633
611.1265
10344637
204.9081
10344653
15.6722
10344658
165.0409
10344674
9.2573
10344679
44.8285
10344707
248.9123
10344713
1241.206
10344723
183.841
10344725
37.476
10344741
2400.4827
10344743
27.1465
10344750
578.7903
10344772
32.4425
10344789
209.6016
10344797
266.8715
10344799
250.4244
24922 423 Kbytes .Supplementary file Size Download File type/resource GSM854335_EA07068_110652_MoGene_T.ETP.TH_6.CEL.gz
4.6 Mb
(ftp) (http) CEL
Processed data included within Sample table
