|
| Status |
Public on Jan 11, 2012 |
| Title |
non-transgenic_B.theta_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
non-transgenic (nTg) rats _B.theta (VPI-5482)
|
| Organism |
Bacteroides thetaiotaomicron |
| Characteristics |
strain/isolate: fully-sequenced human fecal isolate of B.theta (VPI-5482)
host: germ-free HLA-B27/b2 microglobulin non-transgenic (wild type) rat
|
| Growth protocol |
Brain-Heart Infusion (BHI) agar and in BHI broth under strict anaerobic conditions using pre-reduced media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix human gut microbiota community GeneChip, then washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
nTg3
Gene expression data from human fecal isolate of Bacteroides Thetaiotaomicron
|
| Data processing |
Invariantset normalization by dChip software; 4995 probe sets targeting B. Theta transcripts were processed and displayed.
|
| |
|
| Submission date |
Jan 10, 2012 |
| Last update date |
Jan 11, 2012 |
| Contact name |
Yong Huang |
| E-mail(s) |
yh9fj@virginia.edu
|
| Phone |
(434) 243-0842
|
| Organization name |
University of Viginia
|
| Department |
Medicine
|
| Street address |
1340 Jefferson Park Ave
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL7006 |
| Series (1) |
| GSE34966 |
Chronic colitis in Bacteroides Thetaiotaomicron-Monoassociated HLA-B27 Transgenic rats alters expression of receptor and metabolic genes in luminal bacteria. |
|
