|
| Status |
Public on May 19, 2012 |
| Title |
monocytes of Donor # D2 at time point (hour)24 cultured in presence of GMCSF |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
monocytes of Donor # D2 at time point (hour)24 cultured in presence of GMCSF
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: monocytes
donor: D2
hour: 24
cytokine: GMCSF
|
| Growth protocol |
Elutriated monocytes were cultured in RPMI-1640 media with 10% heat inactivated human AB plasma, gentamycin and one among GM-CSF, IL-4, 15 kDa Granulysin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy kit from Qiagen
|
| Label |
Cy5
|
| Label protocol |
Test RNA and Universal Reference RNA () were amplified and labeled according to the Two-color Microarray-based Gene Expression Analysis (quick Amp Labeling) protocol from Agilent Version 5.7
|
| |
|
| Channel 2 |
| Source name |
Universal Human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
origin: Stratagene Catalog #740000
|
| Growth protocol |
Elutriated monocytes were cultured in RPMI-1640 media with 10% heat inactivated human AB plasma, gentamycin and one among GM-CSF, IL-4, 15 kDa Granulysin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy kit from Qiagen
|
| Label |
Cy3
|
| Label protocol |
Test RNA and Universal Reference RNA () were amplified and labeled according to the Two-color Microarray-based Gene Expression Analysis (quick Amp Labeling) protocol from Agilent Version 5.7
|
| |
|
| |
| Hybridization protocol |
Samples were hybridized according to the Two-color Microarray-based Gene Expression Analysis (quick Amp Labeling) protocol from Agilent Version 5.7
|
| Scan protocol |
Scanned on an Agilent G2565BA scanner.
Images were quantified using Agilent Feature Extraction Software
|
| Data processing |
Agilent Feature Extraction Software was used with dafault settings for processed signals
|
| |
|
| Submission date |
Jan 24, 2012 |
| Last update date |
May 20, 2012 |
| Contact name |
Luciano Castiello |
| E-mail(s) |
castiellol@cc.nih.gov
|
| Phone |
3014356380
|
| Fax |
3014358643
|
| Organization name |
NIH/CC
|
| Department |
DTM
|
| Lab |
CPS
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE35304 |
Comparison of the effects of GMCSF, IL-4 and 15kDa Granulysin on elutriated monocytes: a 48 hour time point analysis |
|
