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Status
Public on May 01, 2006
Title
Hoxa11, Hoxd11 null E13.5 kidney rep 1 (430A)
Sample type
RNA
Source name
Hoxa11, Hoxd11 null E13.5 kidneys replicate 1
Organism
Mus musculus Characteristics
Hoxa11, Hoxd11 double null targeted embryonic kidney at E13.5, mixed C57, C3H and CF1 strain, whole kidney
Extracted molecule
total RNA
Extraction protocol
Both kidneys were cleanly dissected from an E13.5 HoxA11/D11 targeted null embryo, rinsed in PBS, total RNA was prepared using the Stratagene Absolutely RNA Nanoprep Kit (La Jolla, CA)
Label
biotin
Label protocol
30 ng of total RNA underwent two round of in vitro transcription according to Baugh LR, Hill AA, Brown EL, Hunter CP: Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic Acids Res 29:E2938, 2001
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Hoxa11, Hoxd11 double null targeted complete (whole) E13.5 kidney replicate 1
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 12, 2005
Last update date
Dec 12, 2005
Contact name
Kristopher Robert Schwab
E-mail(s)
kristopher.schwab@cchmc.org Organization name
Cincinnati Children's Hospital Research Foundation
Department
Developmental Biology
Lab
S. Steven Potter
Street address
3333 Burnett Ave
City
Cincinnati
State/province
OH
ZIP/Postal code
45229
Country
USA
Platform ID
GPL339 Series (1)
GSE3808 Complete embryonic metanephric kidney analysis of wild type and Hoxa11, Hoxd11 null targeted mice
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
414.7
P
0.009337
AFFX-BioB-M_at
528.4
P
0.000225
AFFX-BioB-3_at
244.6
P
0.002023
AFFX-BioC-5_at
845.7
P
0.000225
AFFX-BioC-3_at
553.8
P
0.000052
AFFX-BioDn-5_at
863.1
P
0.000095
AFFX-BioDn-3_at
4830.5
P
0.000195
AFFX-CreX-5_at
8104.7
P
0.000044
AFFX-CreX-3_at
16688.3
P
0.000044
AFFX-DapX-5_at
58.6
A
0.195266
AFFX-DapX-M_at
31.2
A
0.227636
AFFX-DapX-3_at
6.7
A
0.891021
AFFX-LysX-5_at
4.6
A
0.783476
AFFX-LysX-M_at
11
A
0.699394
AFFX-LysX-3_at
18.3
A
0.250796
AFFX-PheX-5_at
13.1
A
0.883887
AFFX-PheX-M_at
6.3
A
0.891021
AFFX-PheX-3_at
80.9
A
0.354453
AFFX-ThrX-5_at
17.6
A
0.712257
AFFX-ThrX-M_at
40.9
A
0.175328
22690 630 Kbytes .Supplementary file Size Download File type/resource GSM87382.CEL.gz
3.6 Mb
(ftp) (http) CEL
GSM87382.EXP.gz
504 b
(ftp) (http) EXP
