|
Status |
Public on Mar 19, 2012 |
Title |
dcr1D_1 |
Sample type |
RNA |
|
|
Source name |
dcr1D_1
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
strain: SPB94 genotype/variation: dcr1D
|
Growth protocol |
Strains grown at 30 degress in YES medium to OD600=0.5
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the hot phenol method
|
Label |
biotin
|
Label protocol |
200ng total RNA was processed according to the GeneChip Whole Transcript (WT) Double-Stranded Target Assay Manual from Affymetrix (GeneChip Whole Transcription Sense Target Labeling technical manual, Rev. 2).
|
|
|
Hybridization protocol |
hybridization was performed for 16h at 45°C and 60rpm in an Affymetrix hybridization oven; washing steps were done in an Affymetrix Fluidics station with the Affymetrix protocol FS450_0002
|
Scan protocol |
GeneChips were scanned with Affymetrix GCC Scan Control v. 3.0.0.1214 on a GeneChip® Scanner 3000 with autoloader.
|
Data processing |
oligo-level data was rma background corrected, quantile normalized and log2 transformed using Bioconductor packages tilingArray and preprocessCore on R
|
|
|
Submission date |
Feb 27, 2012 |
Last update date |
Mar 19, 2012 |
Contact name |
Katrina Woolcock |
Organization name |
Friedrich Miescher Institute for Biomedical Research
|
Lab |
Bühler
|
Street address |
Maulbeerstrasse 66
|
City |
Basel |
ZIP/Postal code |
4058 |
Country |
Switzerland |
|
|
Platform ID |
GPL7715 |
Series (2) |
GSE36113 |
RNA expression for dcr1D, rdp1D, ago1D and swi6D |
GSE36214 |
RNA interference keeps Atf1-bound stress response genes in check at nuclear pores |
|