|
| Status |
Public on Feb 17, 2014 |
| Title |
KL3_muscle_control |
| Sample type |
RNA |
| |
|
| Source name |
Muscle_control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue source: matched healthy control
tissue: Vastus lateralis muscle
tissue state: basal state
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from muscle biopsies using the TRIzol protocol including an extra phenol-chloroform step
|
| Label |
Biotin
|
| Label protocol |
total RNA was converted to amplified RNA (aRNA) and fragmented using the MessageAmpTM III RNA amplification kit
|
| |
|
| Hybridization protocol |
Fragmented aRNA was hybridized to Affymetrix HG-U133A 2.0 chips
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Data were analyzed in R and analyzed using variance stabilizing normalization and model based index calculation (MBEI)
|
| |
|
| Submission date |
Mar 06, 2012 |
| Last update date |
Feb 18, 2014 |
| Contact name |
Vibe Skov |
| E-mail(s) |
vihs@regionsjaelland.dk
|
| Phone |
+4526178735
|
| Organization name |
Roskilde Hospital
|
| Department |
Hematology
|
| Street address |
Koegevej 7-13
|
| City |
Roskilde |
| ZIP/Postal code |
4000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE36297 |
A PGC-1alpha-dependent decrease in mitochondrial oxidative metabolism in muscle of humans with inherited insulin resistance |
|
