|
| Status |
Public on Mar 07, 2012 |
| Title |
Thalassemia kidney vs control kidney Hyb 4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
control
|
| Organism |
Mus musculus |
| Characteristics |
tissue: kidney
strain: control
|
| Growth protocol |
a mouse model of beta-thalassemia was generated by deletion of both b-globin genes, beta-major and beta-minor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Qiagen RNeasy following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
500 ng of total RNA was amplified using Low RNA Input Linear Amp Kit PLUS, One-color. This labeling reaction produces 0.25-2.0 ug of labeled cRNA, by first converting mRNA primed with an oligo (d)T-T7 primer into dsDNA with MMLV-RT and then amplifying the sample using T7 RNA polymerase.
|
| |
|
| Channel 2 |
| Source name |
Th3
|
| Organism |
Mus musculus |
| Characteristics |
tissue: kidney
strain: Th3
|
| Growth protocol |
a mouse model of beta-thalassemia was generated by deletion of both b-globin genes, beta-major and beta-minor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Qiagen RNeasy following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
500 ng of total RNA was amplified using Low RNA Input Linear Amp Kit PLUS, One-color. This labeling reaction produces 0.25-2.0 ug of labeled cRNA, by first converting mRNA primed with an oligo (d)T-T7 primer into dsDNA with MMLV-RT and then amplifying the sample using T7 RNA polymerase.
|
| |
|
| |
| Hybridization protocol |
1.65 ug of purified cRNA was fragmented and hybridized to the array for 17 hr at 65 oC.
|
| Scan protocol |
Array were scanned on an Agilent G2565BA scanner, and analyzed with Agilent Feature Extraction 9.5.
|
| Description |
Thalassemia kidney vs control kidney Hyb 4
|
| Data processing |
"SAM" statistical analysis package version 3.1 was used for data analysis.
|
| |
|
| Submission date |
Mar 06, 2012 |
| Last update date |
Mar 07, 2012 |
| Contact name |
Hanako Kobayashi |
| E-mail(s) |
hanako.kobayashi@vanderbilt.edu
|
| Organization name |
Vanderbilt University
|
| Street address |
1161 21st Ave. MCN C3107
|
| City |
Nashville |
| ZIP/Postal code |
37209 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE36312 |
control vs. Th3 beta thalassemia kidney |
|
