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Sample GSM892245 Query DataSets for GSM892245
Status Public on Dec 31, 2012
Title SiRNA knockdown 2 [CW2011082537]
Sample type RNA
 
Source name ZNF335 knockdown of cells at E14.5 harvested at P0
Organism Mus musculus
Characteristics developmental stage: E15.5
strain: C57BL/6
time point: short term (1 day post knockdown)
Extracted molecule total RNA
Extraction protocol Qiagen total RNA kit
Label biotin
Label protocol labeling of RNA was done with biotinylated ribonucleotide according to Genechip 3 IVT Express Kit User Manual
 
Hybridization protocol hybridized at 45C for 16hours according to Genechip 3 IVT Express Kit User Manual
Scan protocol Affymetrix Genechip scanner 3000 to scan the arrays
Description Gene expression data from cells that show a decrease in ZNF335
short term
Data processing D chip analysis
 
Submission date Mar 09, 2012
Last update date Dec 31, 2012
Contact name Christopher A Walsh
Organization name Children's Hospital Boston
Department Genetics
Street address 3 Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (2)
GSE36384 ZNF335 regulates stem cell proliferation and neuronal differentiation via Trithorax complex and REST/NRSF [gene expression]
GSE36386 ZNF335 regulates stem cell proliferation and neuronal differentiation via Trithorax complex and REST/NRSF

Data table header descriptions
ID_REF
VALUE dChip
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 2106.65 P
AFFX-BioB-M_at 3185.53 P
AFFX-BioB-3_at 3074.48 P
AFFX-BioC-5_at 4716.45 P
AFFX-BioC-3_at 4715.46 P
AFFX-BioDn-5_at 8894.07 P
AFFX-BioDn-3_at 12085.5 P
AFFX-CreX-5_at 23780.66 P
AFFX-CreX-3_at 21242.98 P
AFFX-DapX-5_at 47.47 A
AFFX-DapX-M_at 74.78 A
AFFX-DapX-3_at 30.67 A
AFFX-LysX-5_at 11.5 A
AFFX-LysX-M_at 61.22 A
AFFX-LysX-3_at 34.96 A
AFFX-PheX-5_at 14.29 A
AFFX-PheX-M_at 9.61 A
AFFX-PheX-3_at 107 A
AFFX-ThrX-5_at 18.6 A
AFFX-ThrX-M_at 27.36 A

Total number of rows: 45101

Table truncated, full table size 874 Kbytes.




Supplementary file Size Download File type/resource
GSM892245_CW2011082537.CEL.gz 3.9 Mb (ftp)(http) CEL
GSM892245_CW2011082537.EXP.gz 365 b (ftp)(http) EXP
Processed data included within Sample table

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