|
Status |
Public on Dec 31, 2012 |
Title |
SiRNA knockdown 2 [CW2011082537] |
Sample type |
RNA |
|
|
Source name |
ZNF335 knockdown of cells at E14.5 harvested at P0
|
Organism |
Mus musculus |
Characteristics |
developmental stage: E15.5 strain: C57BL/6 time point: short term (1 day post knockdown)
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen total RNA kit
|
Label |
biotin
|
Label protocol |
labeling of RNA was done with biotinylated ribonucleotide according to Genechip 3 IVT Express Kit User Manual
|
|
|
Hybridization protocol |
hybridized at 45C for 16hours according to Genechip 3 IVT Express Kit User Manual
|
Scan protocol |
Affymetrix Genechip scanner 3000 to scan the arrays
|
Description |
Gene expression data from cells that show a decrease in ZNF335 short term
|
Data processing |
D chip analysis
|
|
|
Submission date |
Mar 09, 2012 |
Last update date |
Dec 31, 2012 |
Contact name |
Christopher A Walsh |
Organization name |
Children's Hospital Boston
|
Department |
Genetics
|
Street address |
3 Blackfan Circle
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE36384 |
ZNF335 regulates stem cell proliferation and neuronal differentiation via Trithorax complex and REST/NRSF [gene expression] |
GSE36386 |
ZNF335 regulates stem cell proliferation and neuronal differentiation via Trithorax complex and REST/NRSF |
|