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Series GSE36384 Query DataSets for GSE36384
Status Public on Dec 31, 2012
Title ZNF335 regulates stem cell proliferation and neuronal differentiation via Trithorax complex and REST/NRSF [gene expression]
Organism Mus musculus
Experiment type Expression profiling by array
Summary The progression from stem cell to differentiated neuron is associated with extensive chromatin remodeling that controls gene expression, but the mechanisms that connect chromatin to gene expression are not well defined. Here we show that mutation of ZNF335 causes severe human microcephaly ("small brain"), small somatic size, and neonatal death. Germline Znf335 null mutations are embryonically lethal in mice, whereas RNA-interference studies and postmortem human studies show that Znf335 is essential for neural progenitor self-renewal, neurogenesis, and neuronal differentiation. Znf335 is a component of a vertebrate-specific, trithorax H3K4 methylation complex, while global ChIP-seq and mRNA expression studies show that Znf335 is a previously unsuspected, direct regulator of REST/NRSF, a master regulator of neural gene expression and neural cell fate, as well as other essential neural-specific genes. Our results reveal ZNF335 as an essential link between H3K4 complexes and REST/NRSF, and provide the first direct evidence that this pathway regulates human neurogenesis and neuronal differentiation.
Overall design ShRNA knockdown cells were FACS sorted and analyzed for changes in gene expression
Contributor(s) Yang YJ, Walsh CA
Citation(s) 23178126
Submission date Mar 09, 2012
Last update date Feb 11, 2019
Contact name Christopher A Walsh
Organization name Children's Hospital Boston
Department Genetics
Street address 3 Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM892244 SiRNA knockdown 1 [CW2011082536]
GSM892245 SiRNA knockdown 2 [CW2011082537]
GSM892246 SiRNA knockdown 3 [CW2011082538]
This SubSeries is part of SuperSeries:
GSE36386 ZNF335 regulates stem cell proliferation and neuronal differentiation via Trithorax complex and REST/NRSF
BioProject PRJNA163273

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36384_RAW.tar 47.9 Mb (http)(custom) TAR (of CEL, EXP)
Processed data included within Sample table

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