|
| Status |
Public on Dec 31, 2012 |
| Title |
SiRNA knockdown 3 [CW2011082538] |
| Sample type |
RNA |
| |
|
| Source name |
ZNF335 knockdown of cells at E14.5 harvested at P0
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E15.5
strain: C57BL/6
time point: short term (1 day post knockdown)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen total RNA kit
|
| Label |
biotin
|
| Label protocol |
labeling of RNA was done with biotinylated ribonucleotide according to Genechip 3 IVT Express Kit User Manual
|
| |
|
| Hybridization protocol |
hybridized at 45C for 16hours according to Genechip 3 IVT Express Kit User Manual
|
| Scan protocol |
Affymetrix Genechip scanner 3000 to scan the arrays
|
| Description |
Gene expression data from cells that show a decrease in ZNF335
short term
|
| Data processing |
D chip analysis
|
| |
|
| Submission date |
Mar 09, 2012 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Christopher A Walsh |
| Organization name |
Children's Hospital Boston
|
| Department |
Genetics
|
| Street address |
3 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE36384 |
ZNF335 regulates stem cell proliferation and neuronal differentiation via Trithorax complex and REST/NRSF [gene expression] |
| GSE36386 |
ZNF335 regulates stem cell proliferation and neuronal differentiation via Trithorax complex and REST/NRSF |
|
