|
| Status |
Public on Mar 24, 2006 |
| Title |
Grown under 150 μM initial iron condition |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Grown under 150 μM initial iron condition
|
| Organism |
Paramagnetospirillum magneticum AMB-1 |
| Characteristics |
Magnetospirillum magneticum AMB-1 (ATCC #700264) grown under 150 μM initial iron condition.
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| |
|
| Channel 2 |
| Source name |
Grown under 0.1 μM initial iron condition
|
| Organism |
Paramagnetospirillum magneticum AMB-1 |
| Characteristics |
Magnetospirillum magneticum AMB-1 (ATCC #700264) grown under 0.1 μM initial iron condition.
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| |
| Description |
Five micrograms of purified RNA was reverse transcribed using random 6-mer primers and the fluorochromes Cy3 dUTP and Cy5 dUTP. Cy3–labeled cDNAs (from mRNA of cells grown under several iron condition) and Cy5–labeled cDNAs (from mRNA of cells grown under 0.1 μM iron: reference condition). Deatails of the sample and experiment can be found in the publications Suzuki et al., The Journal of Bacteriology “Global gene expression analysis of iron-inducible genes in Magnetospirillum magneticum AMB-1”.
|
| Data processing |
Mean ratios of normalized intensities defined as ch1 divided by ch2.
|
| |
|
| Submission date |
Dec 27, 2005 |
| Last update date |
Mar 24, 2006 |
| Contact name |
Tadashi Matsunaga |
| E-mail(s) |
yokamura@cc.tuat.ac.jp
|
| Phone |
+81-42-388-7020
|
| Fax |
+81-42-385-7713
|
| Organization name |
Tokyo University of Agriculture and Technology
|
| Department |
Biotechnology
|
| Lab |
Biochemistry(Matsunaga Lab.)
|
| Street address |
2-24-16
|
| City |
Koganei |
| State/province |
Tokyo |
| ZIP/Postal code |
184-8588 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3072 |
| Series (1) |
| GSE3914 |
Global gene expression analysis of iron-inducible genes in Magnetospirillum magneticum AMB-1 |
|
