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Sample GSM89629 Query DataSets for GSM89629
Status Public on Mar 24, 2006
Title Grown under 200 μM initial iron condition
Sample type RNA
 
Channel 1
Source name Grown under 200 μM initial iron condition
Organism Paramagnetospirillum magneticum AMB-1
Characteristics Magnetospirillum magneticum AMB-1 (ATCC #700264) grown under 200 μM initial iron condition.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name Grown under 0.1 μM initial iron condition
Organism Paramagnetospirillum magneticum AMB-1
Characteristics Magnetospirillum magneticum AMB-1 (ATCC #700264) grown under 0.1 μM initial iron condition.
Extracted molecule total RNA
Label Cy5
 
 
Description Five micrograms of purified RNA was reverse transcribed using random 6-mer primers and the fluorochromes Cy3 dUTP and Cy5 dUTP. Cy3–labeled cDNAs (from mRNA of cells grown under several iron condition) and Cy5–labeled cDNAs (from mRNA of cells grown under 0.1 μM iron: reference condition). Deatails of the sample and experiment can be found in the publications Suzuki et al., The Journal of Bacteriology “Global gene expression analysis of iron-inducible genes in Magnetospirillum magneticum AMB-1”.
Data processing Mean ratios of normalized intensities defined as ch1 divided by ch2.
 
Submission date Dec 27, 2005
Last update date Mar 24, 2006
Contact name Tadashi Matsunaga
E-mail(s) yokamura@cc.tuat.ac.jp
Phone +81-42-388-7020
Fax +81-42-385-7713
Organization name Tokyo University of Agriculture and Technology
Department Biotechnology
Lab Biochemistry(Matsunaga Lab.)
Street address 2-24-16
City Koganei
State/province Tokyo
ZIP/Postal code 184-8588
Country Japan
 
Platform ID GPL3072
Series (1)
GSE3914 Global gene expression analysis of iron-inducible genes in Magnetospirillum magneticum AMB-1

Data table header descriptions
ID_REF
Fold change Mean ratios of normalized intensities defined as ch1 divided by ch2.
VALUE Fold change was converted to Log2

Data table
ID_REF Fold change VALUE
1 2.48 1.310340121
2 2.8 1.485426827
3 1.12 0.163498732
4 1.3 0.378511623
5 0.94 -0.089267338
6 1.71 0.773996325
7 2.28 1.189033824
8 1.23 0.298658316
9 1.24 0.310340121
10 1.36 0.443606651
11 1.48 0.565597176
12 1.42 0.50589093
13 0.99 -0.01449957
14 0.35 -1.514573173
15 1.79 0.839959587
16 2.25 1.169925001
17 1.52 0.604071324
18 2.51 1.327687364
19 1.9 0.925999419
20 1.92 0.941106311

Total number of rows: 4593

Table truncated, full table size 87 Kbytes.




Supplementary data files not provided

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