|
| Status |
Public on Jun 01, 2012 |
| Title |
R108R_2 with array type Medicago from Affymetrix |
| Sample type |
RNA |
| |
|
| Source name |
R108R_2
|
| Organism |
Medicago truncatula |
| Characteristics |
tissue: roots
genotype/variation: wild type
developmental stage (boyes et al. plant cell 2001): roots from 18 days seedling
|
| Treatment protocol |
Name:18 days - induction - infection with symbiotic bacteria,sinorhizobium meliloti
|
| Growth protocol |
roots - - in vitro growth condition on BNM (nitrogen poor medium) plates. - 16h light - 24°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
R108R_2:10ng.
|
| Label |
Biotin
|
| Label protocol |
labelling Biotin direct, amplification=yes, cRNA 20 ug.
|
| |
|
| Hybridization protocol |
labelled extract quantity: 15ug
|
| Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
| Description |
Legume plants establish a symbiotic interaction with soil bacteria called rhizobia. A complex molecular dialogue between the two partners is necessary for the successful infection and organogenesis processes that will ultimately result in the formation of a nitrogen fixing nodule. During a M. truncatula Tnt1 mutant screen, a new class of symbiotic mutant called noot (nodule root) was identified. This original single recessive mutant develops a root in apical position of nodules that are colonized by bacteria and functional for nitrogen fixation. This conversion suggests that the legume nodule morphogenetic pathway may be derived from a root program. We cloned the NOOT gene and showed that it corresponds to Pisum sativum COCH (Ferguson and Reid, 2005, Plant Cell Physiol 46, 1583-1589). The goal of this project is to compare the transcriptomes from wild type nodules and roots to the noot nodule one. This will allow us to unravel the similarity and differences between the wild type and mutant nodule transcriptomes but also to know which developmental pathway is under the control of the NOOT gene.
|
| Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
|
| |
|
| Submission date |
Mar 16, 2012 |
| Last update date |
Jun 01, 2012 |
| Contact name |
stephanie huguet |
| E-mail(s) |
huguet@evry.inra.fr
|
| Organization name |
inra
|
| Street address |
2 rue gaston cremieux
|
| City |
evry |
| ZIP/Postal code |
91000 |
| Country |
France |
| |
|
| Platform ID |
GPL4652 |
| Series (1) |
| GSE36558 |
affy_med_2011_06-Analysis of the Medicago noot mutant |
|
