|
| Status |
Public on Apr 25, 2012 |
| Title |
GFPox |
| Sample type |
RNA |
| |
|
| Source name |
GFPox
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
treatment: none
expression: p35S:GFP-overexpressing
tissue: leaf
|
| Treatment protocol |
Thirty-two-day-old plants were used for analysis. Rosette leaves were harvested from transgenic Arabidopsis plants at ZT0.
|
| Growth protocol |
Plants were grown in soil under long-day (16-h light/8-h dark) conditions at 22°C under white light (90–100 μmol m-2 sec-1).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using the RNeasy Plant mini kit (QIAGEN) following the manufacturer's instruction.
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA was prepared from 100 ng of each total RNA using the Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer's instructions. Dye incorporation and cRNA yield were checked with the ND-1000 Spectrophotometer (NanoDrop Technologies).
|
| |
|
| Hybridization protocol |
The hybridization procedure was performed according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit (Agilent Technologies). 1.65 μg Cy3-labeled fragmented cRNA in hybridization buffer was hybridized overnight (17 hours, 65 °C) to Agilent Whole Arabidopsis Genome Oligo Microarrays 4x44K V4 (G2519F) using Agilent’s recommended hybridization chamber and oven. After hybridization, microarrays were washed once with the Agilent Gene Expression Wash Buffer 1 for 1 min at room temperature followed by a second wash with preheated Agilent Gene Expression Wash Buffer 2 (37 °C) for 1 min. The last washing step was performed with acetonitrile.
|
| Scan protocol |
Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (Agilent Technologies).
|
| Description |
Gene expression data from p35S:GFP-overexpressing leaves
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software (Agilent Technologies).
|
| |
|
| Submission date |
Apr 11, 2012 |
| Last update date |
Apr 25, 2012 |
| Contact name |
Yuji Miyazaki |
| Organization name |
Gakushuin University
|
| Department |
Graduate School of Science
|
| Lab |
Kiyosue Lab
|
| Street address |
1-5-1, mejiro, Toshima-ku
|
| City |
Tokyo |
| ZIP/Postal code |
161-8588 |
| Country |
Japan |
| |
|
| Platform ID |
GPL9020 |
| Series (1) |
| GSE37197 |
Transcriptome analysis of Arabidopsis plants that overexpress green fluorescent protein (GFP) and GFP-LOV kelch protein 2 (GFP-LKP2) |
|
