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Sample GSM915770 Query DataSets for GSM915770
Status Public on Apr 17, 2012
Title PB_DON102
Sample type RNA
 
Source name Normal PB
Organism Homo sapiens
Characteristics diagnosis: healthy control
cell type: Cryopreserved MNCs
Extracted molecule total RNA
Extraction protocol Total RNA extracted using TRIzol reagent (Invitrogen, Carlsbad, CA)according to manufacturer's protocol.
Label Biotin
Label protocol Target was labeled with Enzo BioArray High Yield RNA Trascript Labeling Kit (Enzo Life Technologies, Farmingdale, NY) according to manufacturer's protocol.
 
Hybridization protocol Hybridization was performed according to Affymetrix protocol.
Scan protocol Arrays were scanned according to Affymetrix protocol.
Description GSM240499
4PB_34_M_DON102
Cryopreserved MNCs
Data processing .DAT files were generated using GCOS 1.2.1 software (Affymetrix). CEL files were generated using MAS 5.0 softare (Affymetrix) with target signals for probe sets scaled to 500. Model-based expression levels were computed from .CEL files using dChip software after normalization by invariant set method.
 
Submission date Apr 16, 2012
Last update date Apr 17, 2012
Contact name Sebastian K Ochsenreither
Organization name Charite Berlin
Department Hematology/Oncology
Street address Hindenburgdamm 30
City Berlin
State/province Berlin
ZIP/Postal code 12203
Country Germany
 
Platform ID GPL96
Series (1)
GSE37307 Aberrant expressed genes in AML
Relations
Reanalysis of GSM240499

Data table header descriptions
ID_REF
VALUE linear normalized signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 206.89
AFFX-BioB-M_at 262.66
AFFX-BioB-3_at 312.83
AFFX-BioC-5_at 490.34
AFFX-BioC-3_at 625.49
AFFX-BioDn-5_at 660.34
AFFX-BioDn-3_at 3541.58
AFFX-CreX-5_at 7461.23
AFFX-CreX-3_at 7684.82
AFFX-DapX-5_at 14.37
AFFX-DapX-M_at 28.06
AFFX-DapX-3_at 12.79
AFFX-LysX-5_at 8.89
AFFX-LysX-M_at 18.01
AFFX-LysX-3_at 6.65
AFFX-PheX-5_at 6
AFFX-PheX-M_at 6.28
AFFX-PheX-3_at 66.81
AFFX-ThrX-5_at 8.37
AFFX-ThrX-M_at 18.6

Total number of rows: 22283

Table truncated, full table size 370 Kbytes.




Supplementary file Size Download File type/resource
GSM915770_4PB_34_M_DON102.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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