|
| Status |
Public on Aug 31, 2012 |
| Title |
MeHg3 (Loess) |
| Sample type |
RNA |
| |
|
| Source name |
female hypothalamus
|
| Organism |
Micropterus salmoides |
| Characteristics |
tissue: hypothalamus
treatment: Hg injection 2.5 µg/g body weight
|
| Extracted molecule |
total RNA |
| Extraction protocol |
STAT60 protocol for total RNA
|
| Label |
Cy3
|
| Label protocol |
Agilent one color protocol for Cy3
|
| |
|
| Hybridization protocol |
Agilent one color protocol; Microarrays were washed the following day according to the supplemental Agilent protocol “Preventing Ozone-Related Problems”. Slides were washed in GE Wash Buffer 1 at room temperature for 1 min, followed by GE Wash Buffer 2 at 37ºC for 1 min, acetonitrile wash at room temperature for 1 min, and Stabilizing and Drying Solution wash at room temperature for 30 s.
|
| Scan protocol |
Microarrays were kept in the dark until scanned at 5 μm at both 10 and 100 PMT (Agilent G2505 B Microarray Scanner). Agilent Feature Extraction Software (v9.5) formed a composite of the two scans and calculated parameters for Extended Dynamic Range.
|
| Data processing |
Normalized signal intensity provided here is LOESS Normalization. Data were also normalized using Quantile for the manuscript.
|
| |
|
| Submission date |
Jun 04, 2012 |
| Last update date |
Aug 31, 2012 |
| Contact name |
Christopher Joseph Martyniuk |
| E-mail(s) |
CMARTYN@UFL.EDU
|
| Organization name |
UF
|
| Department |
Physiological Sciences
|
| Street address |
CEHT Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32611 |
| Country |
USA |
| |
|
| Platform ID |
GPL15650 |
| Series (2) |
| GSE38456 |
Characterizing gene regulatory networks in the brain of largemouth bass inhabiting rivers containing high levels of methyl-mercury (lab study) |
| GSE38474 |
Characterizing gene regulatory networks in the brain of largemouth bass inhabiting rivers containing high levels of methyl-mercury |
|
