|
| Status |
Public on Jun 04, 2013 |
| Title |
Nr32_LFK5 |
| Sample type |
RNA |
| |
|
| Source name |
Col-0 ; replicate 4
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: enriched guard cell
ecotype: Col-0
treatment: 4h >85% relative humidity
|
| Treatment protocol |
low humidity treatment: The soil surface of single potted 6-7 week old Arabidopsis thaliana plants was covered with foil to prevent excessive water evaporiation. The plants were subjected to an air stream of 20 ± 5 % rh in a climate chamber at 22 ± 2 °C and 100 µmol m-2 sec-1 white light. Controls were kept at >80% rh.
ABA-Spray treatment: Leaves were sprayed with 50 µM +/- abscisic acid solution, containing 25 µM biologically active ABA and 0.01% (v/v) Triton X-100.After 4 hours epidermal peels were harvested.
|
| Growth protocol |
Arapidopsis thaliana plants were grown in soil and cultivated in a greenhouse under short-day conditions (8 h light/16 h darkness, 22°C/16°C) with illumination by 100 µmol m-2 sec-1 white light. Samples were collected after 4-5h of illumination.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plant Mini Kit, according to the manufacturers protocol
|
| Label |
Cy3
|
| Label protocol |
one-color microarray-based gene expression analysis protocol provided by Agilent including the one-color RNA spike-in kit (v5.0.1, 2006)
|
| |
|
| Hybridization protocol |
one-color microarray-based gene expression analysis protocol provided by Agilent including the one-color RNA spike-in kit (v5.0.1, 2006)
|
| Scan protocol |
Agilent Microarray Scanner,extended dynamic range (XDR) at high resolution (5 µm)
|
| Description |
enriched guard cell fraction of Arabidopsis thaliana
|
| Data processing |
The data were normalized using quantile-quantile as normalization method
|
| |
|
| Submission date |
Jun 11, 2012 |
| Last update date |
Jun 04, 2013 |
| Contact name |
Susanne Kneitz |
| E-mail(s) |
susanne.kneitz@uni-wuerzburg.de
|
| Phone |
0049931-31 86526
|
| Organization name |
University of Wuerzburg
|
| Department |
Physiological Chemistry I
|
| Street address |
Am Hubland, Biocenter
|
| City |
Wuerzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL9020 |
| Series (1) |
| GSE38639 |
Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs. |
|
