|
| Status |
Public on Jul 01, 2014 |
| Title |
WT TAC |
| Sample type |
RNA |
| |
|
| Source name |
Left ventricles, WT, TAC
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: WT
tissue: left ventricles
gender: male
age: 14 weeks
treatment: transverse aortic constriction (TAC)
average hw/bw: 6.7
|
| Treatment protocol |
For the banding procedure, 10-week-old male mice were anesthetized with a mixture of ketamine (90 mg/kg) and xylazine (10 mg/kg). The aortic arch was carefully exposed, and a 7-0 silk suture was used to tightly constrict the aorta between the brachiocephalic trunk and the left carotid artery over a wire of 0.3mm in diameter is put on the aorta. It is banded with the aorta together and is removed. Sham-operated mice were subjected to the same procedure without aortic banding. The hearts were isolated 4 weeks after the operation. The heart weight/body weight (HW/BW) ratio and cross-sectional area of myocytes within the left ventricular wall accordantly showed a greater than 1.5-fold increase in WT TAC compared to WT sham. KO mice showed significantly less cardiac hypertrophy than WT TAC.
|
| Growth protocol |
Standard breeding was performed until 4 weeks after the operation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were extracted from the left ventricles using ISOGEN II reagent (Nippon Gene).
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl aRNA was synthesized using the Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion #1753). Cye dye coupling and fragmentation were performed according to the protocol supplied by TORAY Industries, Inc.
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shaking (250 rpm). Hybridization buffer and washing protocol were according to the protocol supplied by TORAY Industries, Inc.
|
| Scan protocol |
ScanArray Express (PerkinElmer Japan Co., Ltd.) was used for scanning. Images were quantified using GenePix Pro version 6.0 (Axon Instruments).
|
| Description |
#6
|
| Data processing |
The raw data of each spot was normalized by substitution with the mean intensity of the background signal determined by all blank spots' signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Jun 14, 2012 |
| Last update date |
Jul 01, 2014 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL5642 |
| Series (1) |
| GSE38733 |
Gene expression profile of STIM1 deficiency for cardiac remodeling in response to pressure overload by transverse aortic constriction (TAC) |
|
