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Sample GSM956449 Query DataSets for GSM956449
Status Public on Jul 06, 2012
Title PF_gametocyte D8_delta PfPuf2_Rep2
Sample type RNA
 
Source name Plasmodium falciparum dalta PfPuf2, gametocyte stage III
Organism Plasmodium falciparum 3D7
Characteristics genotype/variation: delta-PfPuf2 mutant
developmental stage: Gametocyte Stage III
phenotype: higher ratio of male/female gametocyte
Growth protocol P. falciparum clone 3D7 and dalta PfPuf2 mutant were maintained as described previously (Trager and Jensen, 1976)
Induction of gametocytogenesis was performed as described (Fivelman et al., 2007). Ganetocytes were harvested at Day 8 (stage III) and Day 12 (stage V)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Trizol reagent ((invirtogen.com)
Label Cy5
Label protocol Labeling was done by Genomics Core Facility of Pennsylvania State Univerity. Amplify 1ug of each sample using the Ambion (Life Technologies) Amino Allyl MessageAmp II aRNA Amplification Kit. Dye couple 15 ug of aRNA with Cy3 or Cy5 (GE Health Care #RPN5661), as appropriate, and subsequently purify according to the Ambion Kit instructions.(AM1753).
 
Hybridization protocol Hybridization was performed by Genomics Core Facility of Pennsylvania State University. Incubate at 95C for 5 minutes, followed by 42C for 5 minutes. Attach appropriate mixer to the array and add hybridizations solution according to the manufacturer’s instructions (Roche NimbleGen). Hybridize with mixing in a MAUI hybridization instrument overnight at 42C.
Scan protocol Scanning was performed by Genomics Core Facility of Pennsylvania State University by using Axon GenePix 4000B.
Description This sample is of Plasmodium falciparum 3D7 with deletion of PfPuf2 at gametocyte stage III. It is the second of three mutant strain biological replicates used in this experiment, each from separate cultures.
Data processing The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in DNASTAR software-ArrayStar 4 (dnastar.com).
 
Submission date Jul 05, 2012
Last update date Jul 06, 2012
Contact name JUN MIAO
E-mail(s) jmiao1@usf.edu
Phone 8139747374
Organization name University of South Florida
Department Internal Medicine
Lab Jun Miao and Liwang Cui
Street address 3720 Spectrum Boulevard, MDC84
City Tampa
State/province Florida
ZIP/Postal code 33612
Country USA
 
Platform ID GPL15768
Series (1)
GSE39124 Expression analysis of Plasmodium falciparum 3D7 delta-PfPuf2 mutant

Data table header descriptions
ID_REF
VALUE RMA-normalized, averaged gene-level signal intensity

Data table
ID_REF VALUE
MAL13P1.106100500001737 4194.044327
MAL13P1.114100500001750 81.83877712
MAL13P1.118100500001754 915.6957964
MAL13P1.119100500001755 5250.236511
MAL13P1.120100500001756 8450.353269
MAL13P1.121100500001759 3289.179275
MAL13P1.126100500001764 769.5699513
MAL13P1.129100500001768 5570.804159
MAL13P1.130100500001776 14385.6822
MAL13P1.131100500001780 261.6602709
MAL13P1.132100500001781 41022.05033
MAL13P1.133100500001782 10421.98662
MAL13P1.134100500001784 3356.786279
MAL13P1.135100500001786 248.0853489
MAL13P1.14100500001566 1730.405202
MAL13P1.146100500001831 291.1277449
MAL13P1.148100500001828 1055.533712
MAL13P1.150100500001822 245.4352386
MAL13P1.16100500001571 188.1156576
MAL13P1.160100500001838 119.5079693

Total number of rows: 5367

Table truncated, full table size 175 Kbytes.




Supplementary file Size Download File type/resource
GSM956449_413330A01_635.pair.gz 1.2 Mb (ftp)(http) PAIR
Processed data included within Sample table

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