|
| Status |
Public on Apr 21, 2014 |
| Title |
miR223KO d21 lung exp1 vs B6 d21 lung exp2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
miR223KO d21 M.tuberculosis infection
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
strain: C57Bl/6
genotype/variation: miR223KO
infection status: d21 M.tuberculosis infection
|
| Treatment protocol |
A Glas-Col inhalation exposure system was employed for M.tuberculosis infection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction of murine lungs were performed using Trizol (Invitrogen), according to manufacturer’s instructions.
|
| Label |
Cy5
|
| Label protocol |
Labelling was perfomed using the miRNA Complete Labeling and Hyb Kit (Agilent Technologies) according supplier's recommendations.
|
| |
|
| Channel 2 |
| Source name |
C57Bl/6 wt d21 M.tuberculosis infection
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
strain: C57Bl/6
genotype/variation: wild type
infection status: d21 M.tuberculosis infection
|
| Treatment protocol |
A Glas-Col inhalation exposure system was employed for M.tuberculosis infection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction of murine lungs were performed using Trizol (Invitrogen), according to manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Labelling was perfomed using the miRNA Complete Labeling and Hyb Kit (Agilent Technologies) according supplier's recommendations.
|
| |
|
| |
| Hybridization protocol |
Labeled samples were mixed and hybridized using 1x Hi hybridization buffer together with blocking buffer according to the supplier`s recommendations (Agilent Technologies). Washing of the hybridized arrays was done with the SSC protocol according supplier`s protocol (Agilent Technologies).
|
| Scan protocol |
Scanning of microarrays was performed with 5 µm resolution and extended range using a DNA microarray laser scanner (Agilent Technologies).
|
| Description |
miRNA expression in lung tissue
|
| Data processing |
Raw microarray image data were analyzed with the Image Analysis / Feature Extraction software G2567AA (Version A.10.5.1) according supplier's recommendations (Agilent Technologies). Data analysis was carried out on the Rosetta Resolver gene expression data management and analysis system (Build 7.2.2.0.SP1.31) on sequence level.
|
| |
|
| Submission date |
Jul 06, 2012 |
| Last update date |
Apr 21, 2014 |
| Contact name |
Hans-Joachim Mollenkopf |
| E-mail(s) |
mollenkopf@mpiib-berlin.mpg.de
|
| Phone |
+49 30 28460 482
|
| Organization name |
Max-Planck-Institute for Infection Biology
|
| Lab |
Microarray/Genomics Core Facility
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE39163 |
MicroRNA miR-223 constrains lethal inflammation during Mycobacterium tuberculosis infection |
|
