|
| Status |
Public on Aug 01, 2012 |
| Title |
CI25E replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from CI25E exponential phase
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genetic variant: CI25E
|
| Growth protocol |
Microarray analysis was performed on wt and cobalt resistant mutants. These cultures were grown in three replicates on 100ml YMM at 500 ml flask to their exponential phase DO600=3 ; 3 107 cells/ml
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from three independant cultures were extracted using the SV Total RNA isolation System (promega)
|
| Label |
Cy-3
|
| Label protocol |
Incorporation of cyanine-3 and cyanine-5 was performed during reverse transcription of total RNA using label star reverse transcriptase (quiagen). Labelled cDNA from the microarray experiment was purified on minElute spin columns (quiagen).
|
| |
|
| Hybridization protocol |
Hybridation was carried out in an automatic hybridization chamber ( Discovery from Ventana Medical System) for 10h at 42°C. After hybridization, slides were washed in 2xSSC/0,2%(v/v)SDS, immersed briefly in iso-propanol and then dried under a stream of air.
|
| Scan protocol |
The hybridization signal were detected by scanning using GenePix 4000B laser Scanner (Axon Instruments) and transformed to numerical values using the integrated GenePix software version 3.01
|
| Description |
CI25E harvested in exponential phase biological replicate 1
|
| Data processing |
R computing environnement was used for background subtraction and weighted global median and quantile normalization was performed
|
| |
|
| Submission date |
Jul 09, 2012 |
| Last update date |
Aug 01, 2012 |
| Contact name |
Laurent Benbadis |
| E-mail(s) |
laurent.benbadis@insa-toulouse.fr
|
| Phone |
+33(0)561559420
|
| Fax |
+33(0)561559400
|
| Organization name |
LISBP INSA CNRS UMR5504
|
| Street address |
135 Av de Rangueil
|
| City |
Toulouse |
| ZIP/Postal code |
31077 CEDEX |
| Country |
France |
| |
|
| Platform ID |
GPL9294 |
| Series (1) |
| GSE39185 |
Transcriptomic analysis of an evolutionary strain of Saccharomyces cerevisiae resistant to cobalt 10mM |
|
