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Sample GSM959962 Query DataSets for GSM959962
Status Public on Jul 07, 2013
Title Metastatic Bladder Cancer 1281
Sample type genomic
 
Source name Metastatic Bladder Cancer
Organism Homo sapiens
Characteristics unique_patient_id: 1281
sample_type: tumor
surgery_type: radical cystectomy
summarystage: invasive
tumor stage: 4
visceral_metastasis: n
ecog_ps (eastern cooperative oncology group performance status): 1
adjuvant_chemo: y
recurrence_status: recurrence
days_to_death: 796
vital_status: living
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted from tumors using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA)
Label ULS-Cy5
Label protocol The Genomic DNA ULS labeling kit for FFPE Samples (Agilent Technologies, Inc., Palo Alto, CA) was used to chemically label 500ng of DNA with either ULS-Cy5 (tumor) or ULS-Cy3 dye (normal/reference DNA) following the manufacturer's protocol
 
Hybridization protocol Genomic DNA samples were hybridized to the array using an Agilent microarray hybridization chamber for 40 hours in a Robbins Scientific oven with rotation at 20 rpm at 65°C
Scan protocol Post-hybridization, the slides were washed and scanned using an Agilent DNA microarray scanner
Description Genomic DNA isolated from metastatic bladder cancer
Data processing CGH Analytics software (version 3.4, Agilent Technologies, CA) was used to analyze the aCGH data
Copy numbers were segmented using the GLAD algorithm. The 'spanishaCGH.glad.txt' are the segmented data (like the Level 3 data in TCGA), calculated with the GLAD algorithm: Sample ID, Chromosome, Start, End, Number of Probes in the segment, log2 copy number ratio of the segment (available on a Series records).
 
Submission date Jul 11, 2012
Last update date Jul 07, 2013
Contact name Markus Riester
E-mail(s) markus@jimmy.harvard.edu
Organization name Dana-Farber Cancer Institute
Department Biostatistics & Computational Biology
Lab Michor
Street address 3 Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL10150
Series (2)
GSE39281 DNA copy number gain at 1q23.3 is associated with poor survival in metastatic bladder cancer [Agilent]
GSE39282 DNA copy number gain at 1q23.3 is associated with poor survival in metastatic bladder cancer

Data table header descriptions
ID_REF
VALUE CopyNumber

Data table
ID_REF VALUE
A_16_P15000916 0.147004677266532
A_18_P10001325 -0.865021761753967
A_18_P10001390 -0.136638511770115
A_18_P10001457 -0.262744259582125
A_18_P10001545 -0.297078549447702
A_16_P15001543 0.285650896245557
A_16_P15001594 0.279251868426365
A_16_P00000099 0.0343170413833986
A_18_P10001772 0.202702814582652
A_16_P00000136 0.0880149180188683
A_16_P15001756 -0.301559254940236
A_16_P00000179 0.254690017522582
A_16_P00000195 0.659292453568831
A_16_P15001886 -0.115683534625734
A_16_P00000237 0.0199490789037633
A_16_P15001967 0.340500923679496
A_16_P00000285 0.168888018895867
A_16_P00000311 0.444192331728686
A_16_P15002151 0.00876342270814945
A_18_P17422640 0.0504668466056304

Total number of rows: 164629

Table truncated, full table size 5354 Kbytes.




Supplementary file Size Download File type/resource
GSM959962_1281.txt.gz 18.5 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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