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Sample GSM959991 Query DataSets for GSM959991
Status Public on Jul 07, 2013
Title Metastatic Bladder Cancer 1513
Sample type genomic
Source name Metastatic Bladder Cancer
Organism Homo sapiens
Characteristics unique_patient_id: 1513
sample_type: tumor
surgery_type: radical cystectomy
summarystage: invasive
tumor stage: 2
visceral_metastasis: n
ecog_ps (eastern cooperative oncology group performance status): 1
adjuvant_chemo: y
recurrence_status: recurrence
days_to_death: 644
vital_status: living
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted from tumors using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA)
Label ULS-Cy5
Label protocol The Genomic DNA ULS labeling kit for FFPE Samples (Agilent Technologies, Inc., Palo Alto, CA) was used to chemically label 500ng of DNA with either ULS-Cy5 (tumor) or ULS-Cy3 dye (normal/reference DNA) following the manufacturer's protocol
Hybridization protocol Genomic DNA samples were hybridized to the array using an Agilent microarray hybridization chamber for 40 hours in a Robbins Scientific oven with rotation at 20 rpm at 65°C
Scan protocol Post-hybridization, the slides were washed and scanned using an Agilent DNA microarray scanner
Description Genomic DNA isolated from metastatic bladder cancer
Data processing CGH Analytics software (version 3.4, Agilent Technologies, CA) was used to analyze the aCGH data
Copy numbers were segmented using the GLAD algorithm. The 'spanishaCGH.glad.txt' are the segmented data (like the Level 3 data in TCGA), calculated with the GLAD algorithm: Sample ID, Chromosome, Start, End, Number of Probes in the segment, log2 copy number ratio of the segment (available on a Series records).
Submission date Jul 11, 2012
Last update date Jul 07, 2013
Contact name Markus Riester
Organization name Dana-Farber Cancer Institute
Department Biostatistics & Computational Biology
Lab Michor
Street address 3 Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL10150
Series (2)
GSE39281 DNA copy number gain at 1q23.3 is associated with poor survival in metastatic bladder cancer [Agilent]
GSE39282 DNA copy number gain at 1q23.3 is associated with poor survival in metastatic bladder cancer

Data table header descriptions
VALUE CopyNumber

Data table
A_16_P15000916 -0.508670134333881
A_18_P10001325 -0.497861921893341
A_18_P10001390 -0.169096811532538
A_18_P10001457 -0.230354255738107
A_18_P10001545 0.215059630945374
A_16_P15001543 0.298188595952388
A_16_P15001594 0.0265059556069818
A_16_P00000099 0.0275922583346939
A_18_P10001772 -0.0427634019052998
A_16_P00000136 0.400949877920991
A_16_P15001756 -0.373293502916132
A_16_P00000179 0.268962373579622
A_16_P00000195 0.617329089868342
A_16_P15001886 0.144940615847978
A_16_P00000237 0.0185831324207664
A_16_P15001967 0.160588297274961
A_16_P00000285 0.0890047141847936
A_16_P00000311 0.498592386758461
A_16_P15002151 -0.0175331934090153
A_18_P17422640 0.045613992577757

Total number of rows: 164629

Table truncated, full table size 5352 Kbytes.

Supplementary file Size Download File type/resource
GSM959991_1513.txt.gz 18.5 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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