|
| Status |
Public on Sep 25, 2012 |
| Title |
WT_Tm_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
WT treated 1h with 1 µg/ml Tunicamycin, replica 1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain background: W303, mat alpha
genotype/variation: Wild type
agent: Tunicamycin
|
| Treatment protocol |
The UPR was induced by the addition of 2 mM DTT or 1 µg/ml Tm for one hour prior to harvest the cells
|
| Growth protocol |
Culture were grown to an OD600 of 0.25 at 30oC in selective synthetic complete media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by the hot acid phenol method as previously described (Spellman et al., 1998). Total RNA was subsequently cleaned up using Rnease Mini Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Labeling System with 100 ng of total RNA as starting material following manufacturer instruction: One-Color Microarray-Based Gene Expression Analysis Protocol version 6.5 (Agilent Technologies)
|
| |
|
| Hybridization protocol |
Hybridized on Custom Microarray Agilent GE 4x44K array
|
| Scan protocol |
Arrays were scanned using High-resolution DNA Microarray Scanner model: G2505C (Agilent Technologies)
|
| Data processing |
Data was analyzed using GeneSpring GX software (Agilent Technologies). Normalization: Percentile shift to 75th percentile (per chip normalization); Baseline to Median of Individual batch data (per gene normalization).
For additional data processing details (eg, settings, filtering, etc.), please see the supplementary file linked to the Series record.
|
| |
|
| Submission date |
Jul 17, 2012 |
| Last update date |
Sep 25, 2012 |
| Contact name |
Guillaume Thibault |
| E-mail(s) |
guillou1976@gmail.com
|
| Organization name |
Temasek Life Sciences Laboratory
|
| Department |
Cell Stress and Homeostasis
|
| Lab |
Davis Ng
|
| Street address |
1 Research Link, National University of Singapore
|
| City |
Singapore |
| ZIP/Postal code |
117604 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL9294 |
| Series (1) |
| GSE39419 |
The Membrane Stress Response Buffers Lethal Effects of Lipid Disequilibrium by Reprogramming the Protein Homeostasis Network |
|
