|
Status |
Public on Apr 25, 2013 |
Title |
HUVEC/Vector_pH 6.4-treated_5 h_rep4 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
HUVECs with endogenous GPR4, pH 6.4-treated, 5 h
|
Organism |
Homo sapiens |
Characteristics |
cell line: HUVEC medium: EGM-2_HEPES-EPPS-MES-buffered, pH 6.4
|
Treatment protocol |
HUVECs with endogenous or overexpressed GPR4 level were treated for 5 h with EGM-2 medium that was buffered with 7.5 mM HEPES, 7.5 mM EPSS & 7.5 mM MES and ajusted to pH 8.4 or 6.4.
|
Growth protocol |
HUVECs with endogenous or overexpressed GPR4 level were grown in endothelial growth medium-2 (EGM-2; Lonza, CC-3162) in 10 cm tissue culture plates to reach 80-90% confluency.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Plus mini kit (QIAGEN, 74134) following manufacterer's instructions.
|
Label |
Cy5
|
Label protocol |
Agilent Quick Amp Labeling Kits
|
|
|
Channel 2 |
Source name |
HUVECs with endogenous GPR4, pH 8.4-treated, 5 h
|
Organism |
Homo sapiens |
Characteristics |
cell line: HUVEC medium: EGM-2_HEPES-EPPS-MES-buffered, pH 8.4
|
Treatment protocol |
HUVECs with endogenous or overexpressed GPR4 level were treated for 5 h with EGM-2 medium that was buffered with 7.5 mM HEPES, 7.5 mM EPSS & 7.5 mM MES and ajusted to pH 8.4 or 6.4.
|
Growth protocol |
HUVECs with endogenous or overexpressed GPR4 level were grown in endothelial growth medium-2 (EGM-2; Lonza, CC-3162) in 10 cm tissue culture plates to reach 80-90% confluency.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Plus mini kit (QIAGEN, 74134) following manufacterer's instructions.
|
Label |
Cy3
|
Label protocol |
Agilent Quick Amp Labeling Kits
|
|
|
|
Hybridization protocol |
Agilent Gene Expression Hybridization Kit
|
Scan protocol |
Scanned on an Agilent Technologies Scanner G2505C US82800149
|
Data processing |
Images were quantified using Agilent Feature Extraction Software (version A.10.7.3.1). Background subtraction and LOWESS normalization (https://genome.unc.edu/)
|
|
|
Submission date |
Aug 12, 2012 |
Last update date |
Apr 25, 2013 |
Contact name |
Lixue Dong |
E-mail(s) |
dongl@ecu.edu
|
Organization name |
East Carolina University
|
Street address |
600 Moye Blvd.
|
City |
Greenville |
State/province |
NC |
ZIP/Postal code |
27834 |
Country |
USA |
|
|
Platform ID |
GPL6480 |
Series (1) |
GSE40060 |
HUVECs with endogenous or overexpressed GPR4 treated with pH 8.4 (control) or pH 6.4 for 5 h |
|