|
| Status |
Public on Apr 25, 2013 |
| Title |
HUVEC/GPR4_pH 6.4-treated_5 h_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HUVECs with overexpressed GPR4, pH 6.4-treated, 5 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HUVEC
medium: EGM-2_HEPES-EPPS-MES-buffered, pH 6.4
|
| Treatment protocol |
HUVECs with endogenous or overexpressed GPR4 level were treated for 5 h with EGM-2 medium that was buffered with 7.5 mM HEPES, 7.5 mM EPSS & 7.5 mM MES and ajusted to pH 8.4 or 6.4.
|
| Growth protocol |
HUVECs with endogenous or overexpressed GPR4 level were grown in endothelial growth medium-2 (EGM-2; Lonza, CC-3162) in 10 cm tissue culture plates to reach 80-90% confluency.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Plus mini kit (QIAGEN, 74134) following manufacterer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Agilent Quick Amp Labeling Kits
|
| |
|
| Channel 2 |
| Source name |
HUVECs with overexpressed GPR4, pH 8.4-treated, 5 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HUVEC
medium: EGM-2_HEPES-EPPS-MES-buffered, pH 8.4
|
| Treatment protocol |
HUVECs with endogenous or overexpressed GPR4 level were treated for 5 h with EGM-2 medium that was buffered with 7.5 mM HEPES, 7.5 mM EPSS & 7.5 mM MES and ajusted to pH 8.4 or 6.4.
|
| Growth protocol |
HUVECs with endogenous or overexpressed GPR4 level were grown in endothelial growth medium-2 (EGM-2; Lonza, CC-3162) in 10 cm tissue culture plates to reach 80-90% confluency.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Plus mini kit (QIAGEN, 74134) following manufacterer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Agilent Quick Amp Labeling Kits
|
| |
|
| |
| Hybridization protocol |
Agilent Gene Expression Hybridization Kit
|
| Scan protocol |
Scanned on an Agilent Technologies Scanner G2505C US82800149
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version A.10.7.3.1).
Background subtraction and LOWESS normalization (https://genome.unc.edu/)
|
| |
|
| Submission date |
Aug 12, 2012 |
| Last update date |
Apr 25, 2013 |
| Contact name |
Lixue Dong |
| E-mail(s) |
dongl@ecu.edu
|
| Organization name |
East Carolina University
|
| Street address |
600 Moye Blvd.
|
| City |
Greenville |
| State/province |
NC |
| ZIP/Postal code |
27834 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE40060 |
HUVECs with endogenous or overexpressed GPR4 treated with pH 8.4 (control) or pH 6.4 for 5 h |
|
