|
| Status |
Public on Mar 11, 2015 |
| Title |
squamous cell carcinoma sample 4 |
| Sample type |
RNA |
| |
|
| Source name |
squamous carcinoma cell
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung
cell type: squamous carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Rneasy Tissue Kit (Qiagen) following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Briefly, 500 ng of total RNA with control RNA Spike-In were amplified and labeled with Cy3-CTP (Agilent Technologies, Santa Clara, California).
|
| |
|
| Hybridization protocol |
Cy3-labeled RNA was hybridized at 65°C for 24 hr. The array was scanned and then analyzed using Agilent Feature Extraction Software (version 10.7.1.1).
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
Images were quantified using Agilent Feature Extraction Software (version 10.7.1.1).
|
| Description |
gene expression
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.1.1) was used for background subtraction.
|
| |
|
| Submission date |
Aug 13, 2012 |
| Last update date |
Mar 12, 2015 |
| Contact name |
Pierre Massion |
| E-mail(s) |
pierre.massion@vanderbilt.edu
|
| Phone |
615-936-2256
|
| Organization name |
Vanderbilt University
|
| Department |
Vanderbilt Ingram Cancer Center
|
| Street address |
2220 Pierce Ave
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE40074 |
Agilent 4x44K expression array for squamous cell carcinoma of the lung |
| GSE40089 |
Squamous cell carcinoma of the lung |
|
