|
Status |
Public on Sep 06, 2012 |
Title |
parotid tissue from control 8 |
Sample type |
RNA |
|
|
Source name |
parotid tissue from control
|
Organism |
Homo sapiens |
Characteristics |
disease status: Control batch: 1
|
Growth protocol |
parotid tissue from humans
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were extracted using mirVana PARIS Kit (Ambion/Applied Biosystems).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA
|
|
|
Hybridization protocol |
15μg of labeled cRNA was fragmented into 50- to 200-bp fragments and assessed for quality with a 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA)
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
Data processing |
Microarray data were processed and normalized using MAS5.
|
|
|
Submission date |
Sep 05, 2012 |
Last update date |
Nov 14, 2016 |
Contact name |
Steve Horvath |
E-mail(s) |
shorvath@altoslabs.com
|
Organization name |
University of California, Los Angeles
|
Department |
Human Genetics
|
Lab |
Horvath
|
Street address |
695 Charles E. Young Drive South, Box 708822
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095-7088 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE40611 |
Gene expression data of parotid tissue from Primary Sjogren’s Syndrome and controls |
|
Relations |
Reanalyzed by |
GSE89827 |