|
| Status |
Public on Sep 01, 2015 |
| Title |
Day2_cntrl_2 |
| Sample type |
RNA |
| |
|
| Source name |
day2, control, replica 2
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mESC, i-Mixl1
treatment: No Doxycycline
time: day 2
|
| Growth protocol |
Differentiating i-Mixl1 ES cells cultured in the presence or absence of Doxycycline (DOX, 0.1 ug/ml, 3 replicates per treatment/time point).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from EBs harvested at day 2, 3 and 4 (DOX added 1 day after plating of ES cells) using RNeasy Mini columns (Qiagen). RNA (1 ug) was subjected to one round of linear amplification (RiboAmp System) to yield 10 ug of RNA
|
| Label |
Cy5
|
| Label protocol |
The RNA was indirectly labeled using amino allyl-dUTP('t Hoen, de Kort et al., 2003, Nucleic Acids Res. 31: e20), then conjugated with Cy3 or Cy5.
|
| |
|
| Hybridization protocol |
The labeled RNAs were used to screen a 15K mouse developmental cDNA microarray(Tanaka, Jaradat et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97: 9127-9132).
|
| Scan protocol |
Hybridized microarrays were scanned using standard procedures (Tanaka, Jaradat et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97: 9127-9132).
|
| Description |
No Doxycycline
|
| Data processing |
Pairwise analysis of hybridization results for EBs cultured with or without DOX was performed for samples harvested on each day. Spotfire(R) software was used for data management and filtering.
|
| |
|
| Submission date |
Sep 07, 2012 |
| Last update date |
Sep 01, 2015 |
| Contact name |
Dmitri Papatsenko |
| E-mail(s) |
dmitri.papatsenko@gmail.com
|
| Organization name |
Mount Sinai School of Medicine
|
| Street address |
One Gustave L. Levy Place
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029-6574 |
| Country |
USA |
| |
|
| Platform ID |
GPL236 |
| Series (1) |
| GSE40703 |
Differentiating i-Mixl1 mESCs cultured in the presence or absence of Doxycycline |
|
