Heteroduplex Analysis

A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.

Year introduced: 1999

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Tree Number(s): E05.393.661.250

MeSH Unique ID: D020180

Entry Terms:

• Analyses, Heteroduplex
• Analysis, Heteroduplex
• Heteroduplex Analyses

Previous Indexing:

• Nucleic Acid Heteroduplexes (1980-1998)
• Nucleic Acid Hybridization (1973-1979)

See Also:

• Nucleic Acid Heteroduplexes
• Base Pair Mismatch

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Genetic Techniques
Nucleic Acid Hybridization
Heteroduplex Analysis