GEO DataSets

(Submitter supplied) Chlamydomonas reinhardtii provides an important model to study sexual reproduction in a unicellular context. Its sexual cycle culminates in the formation of diploid zygotes which differentiate into dormant spores that eventually undergo meiosis. Mating between haploid plus and minus gametes induces rapid cell wall shedding via the enzyme g-Lysin; cell fusion is followed by by heterodimerization of sex-specific homeobox transcription factors, Gsm1 and Gsp1, and initiation of zygote-specific gene expression. more...

Organism:

Chlamydomonas reinhardtii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15083

8 Samples

Download data: DIFF, FPKM_TRACKING, TXT

(Submitter supplied) Phosphorus (P) is an essential nutrient that is limiting in many environments. When P is scarce organisms employ strategies for conservation of internal stores, and to efficiently scavenge P from their external surroundings. In this study we investigated the acclimation response of Chlamydomonas reinhardtii to P deficiency, comparing the transcriptional profiles of P starved wild-type cells to the P replete condition. more...

Organism:

Chlamydomonas reinhardtii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15083

2 Samples

Download data: TXT

(Submitter supplied) To understand the molecular basis underlying increased triacylglycerol accumulation in starchless Chlamydomonas mutants, we undertook comparative time course transcriptomics of sta6 (CC-4348), a cw strain purported to represent the parental STA6 strain (CC-4349), and 3 independent STA6 strains (STA6-C2, STA6-C4 and STA6-C6, generated by complementation of sta6, CC-4565, –4566 and –4567) in the context of nitrogen deprivation. more...

Organism:

Chlamydomonas reinhardtii

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL17828 GPL15083

31 Samples

Download data: TXT

(Submitter supplied) We used pH shock to deflagellate Chlamydomonas and collected samples before pH shock (pre), 3, 10, 30, and 60 min post-deflagellation to look at the genes that are upregulated as Chlamydomonas regrows its flagella. We aligned our data to v5.3 of the Chlamydomonas genome and compared transcript levels post-deflagellation to the pre-deflagellation levels. A gene was considered expressed if the FPKMM value was at least 3. more...

Organism:

Chlamydomonas reinhardtii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15083

5 Samples

Download data: TXT

(Submitter supplied) We used RNA-Seq to compare the transcriptomes of Fe-replete vs. Fe-deficient vs. Fe-limited Chlamydomonas wild-type cells. Our RNA-Seq data revealed 90 and 49 genes to be specifically expressed under hetero-phototrophic and phototrophic conditions, respectively. Around 30 genes represent putative Fe-deficiency targets, independent of the carbon source used. Many of these Fe-specific responses are conserved between Chlamydomonas and land plants. more...

Organism:

Chlamydomonas reinhardtii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15083

20 Samples

Download data: BOWTIE

(Submitter supplied) The l-galactose (Smirnoff-Wheeler) pathway represents the major route to l-ascorbic acid (vitamin C) biosynthesis in plants. Arabidopsis thaliana VTC2 and its paralogue VTC5 function as GDP-l-galactose phosphorylases converting GDP-l-galactose to l-galactose-1-P, thus catalyzing the first committed step in the biosynthesis of l-ascorbate. Here we report that the l-galactose pathway of ascorbate biosynthesis described in higher plants is conserved in green algae. more...

Organism:

Chlamydomonas reinhardtii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15083

3 Samples

Download data: BOWTIE