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Comparing gene expression profiles of wild-type and the ∆phcA mutant strains of Ralstonia solanacearum during tomato colonization

(Submitter supplied) Purpose: To comprehensively identify traits controlled by the PhcA quorum sensing system, and to better understand how this regulator affects pathogen behavior in its natural host environment Method: 21 day-old tomato plants were inoculated with approximately 2000 cells of either wild-type Rs strain GMI1000 or ΔphcA through a cut petiole. After 72 h in a 28°C growth chamber (12h day/night cycle), 0.1 g of stem tissue spanning the petiole was harvested and transferred immediately to a tube containing 900 µl of pre-chilled transcription stop solution (5% [vol/vol] water-saturated phenol in ethanol) and ground using a Powerlyzer. more...
Organism:
Ralstonia solanacearum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL23347
6 Samples
Download data: XLSX
Series
Accession:
GSE98074
ID:
200098074

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