GEO DataSets

(Submitter supplied) Illumina sequencing data used in HIV-CRISPR screen with an ISG-specific sgRNA library (PIKAHIV) to find genes that block infection by the N74D and P90A HIV-1 capsid mutant viruses in THP-1 monocytic cells. For more information on the library and approach see Ohainle et al. eLife 2018 (PMID: 30520725). All screens performed here were done in a clonal ZAP-KO cell line (ZAP may inhibit the HIV-CRISPR vector used in the screen). more...

Organism:

Human immunodeficiency virus; Homo sapiens

Type:

Other

Platforms:

GPL16791 GPL25457

12 Samples

Download data: TXT

(Submitter supplied) Interferon (IFN) inhibits HIV replication by inducing an array of antiviral effectors. Here we describe a novel CRISPR knockout screening approach to identify the ensemble of these HIV restriction factors. We assembled a CRISPR sgRNA library specific for Interferon Stimulated Genes (ISGs) into a modified lentiviral vector that allows for packaging of sgRNA-encoding genomes in trans into budding HIV-1 particles. more...

Organism:

Human immunodeficiency virus; Homo sapiens

Type:

Other

Platforms:

GPL25457 GPL16791

18 Samples

Download data: FA, TXT