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Bacterial mRNA sequencing through targeted rRNA depletion for efficient RNA-seq in varied species and co-cultures

(Submitter supplied) mRNA sequencing in bacteria is challenging due to the abundance of ribosomal rRNA that cannot be easily removed prior to sequencing. While commercially available kits target specific rRNA sequences found in defined lists of common bacterial species, they are frequently inefficient when applied to other divergent species, including those from environmental isolates. Similar to the commercial kits, other common techniques for rRNA depletion typically employ large probe sets that tile full-length rRNA sequences; however, such approaches are both time consuming and expensive when applied to multiple species or complex consortia of non-model microbes. more...
Organism:
Caecomyces churrovis; Escherichia coli; Fibrobacter succinogenes; Geobacter metallireducens; Anaeromyces robustus
Type:
Expression profiling by high throughput sequencing
10 related Platforms
65 Samples
Download data: TXT
Series
Accession:
GSE223404
ID:
200223404

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