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Items: 11

1.

Transcriptome-wide mapping of RNA secondary structure ensembles in a living cell

(Submitter supplied) RNA molecules can populate an ensemble of alternative structural conformations, but the true extent of the RNA secondary structure folding space of a living cell has never been explored. We here generated the first transcriptome-wide maps of RNA secondary structure ensembles in Escherichia coli, both at physiological temperature and upon cold-shock. Our analysis revealed hundreds of novel putative conserved RNA switches.
Organism:
Escherichia coli
Type:
Other
Platforms:
GPL33918 GPL33919
10 Samples
Download data: WIG
Series
Accession:
GSE247244
ID:
200247244

2.

Reconstitution of SPO11-dependent double-strand break formation

(Submitter supplied) Homologous meiotic recombination starts with DNA double-strand breaks (DSBs) generated by SPO11 protein. SPO11 is critical for meiosis in most species but the DSBs it makes are also dangerous because of their mutagenic and gametocidal potential, so cells must foster beneficial functions of SPO11 while minimizing its risks. SPO11 mechanism and regulation remain poorly understood. Here we report reconstitution of DNA cleavage in vitro with purified recombinant mouse SPO11 bound to its essential partner TOP6BL. more...
Organism:
Mus musculus; Cloning vector pUC19; Saccharomyces cerevisiae; Escherichia coli
Type:
Other
4 related Platforms
14 Samples
Download data: BED, BW
Series
Accession:
GSE275291
ID:
200275291

3.

NextSeq 1000 (Escherichia coli)

Organism:
Escherichia coli
2 Series
8 Samples
Download data
Platform
Accession:
GPL33919
ID:
100033919

4.

DMS-MaPseq of E. coli TOP10, exponential phase, ex vivo, 37°C (total RNA)

Organism:
Escherichia coli
Source name:
TOP10
Platform:
GPL33919
Series:
GSE247244
Download data: WIG
Sample
Accession:
GSM7885845
ID:
307885845

SRA Run Selector

5.

DMS-MaPseq of E. coli DH5a, exponential phase, ex vivo, 37°C (total RNA)

Organism:
Escherichia coli
Source name:
DH5a
Platform:
GPL33919
Series:
GSE247244
Download data: WIG
Sample
Accession:
GSM7885844
ID:
307885844

SRA Run Selector

6.

DMS-MaPseq of E. coli TOP10, exponential phase, in vivo, 37°C (total RNA)

Organism:
Escherichia coli
Source name:
TOP10
Platform:
GPL33919
Series:
GSE247244
Download data: WIG
Sample
Accession:
GSM7885843
ID:
307885843

SRA Run Selector

7.

DMS-MaPseq of E. coli DH5a, exponential phase, in vivo, 37°C (total RNA)

Organism:
Escherichia coli
Source name:
DH5a
Platform:
GPL33919
Series:
GSE247244
Download data: WIG
Sample
Accession:
GSM7885842
ID:
307885842

SRA Run Selector

8.

TDP2seq_Ecoli_DNA_SPO11_YF_rep2

Organism:
Escherichia coli
Source name:
Mus musculus, Escherichia coli DNA
Platform:
GPL33919
Series:
GSE275291
Download data: BED, BW
Sample
Accession:
GSM8474255
ID:
308474255

SRA Run Selector

9.

TDP2seq_Ecoli_DNA_SPO11_YF_rep1

Organism:
Escherichia coli
Source name:
Mus musculus, Escherichia coli DNA
Platform:
GPL33919
Series:
GSE275291
Download data: BW
Sample
Accession:
GSM8474254
ID:
308474254

SRA Run Selector

10.

TDP2seq_Ecoli_DNA_SPO11_WT_rep2

Organism:
Escherichia coli
Source name:
Mus musculus, Escherichia coli DNA
Platform:
GPL33919
Series:
GSE275291
Download data: BED, BW
Sample
Accession:
GSM8474253
ID:
308474253

SRA Run Selector

11.

TDP2seq_Ecoli_DNA_SPO11_WT_rep1

Organism:
Escherichia coli
Source name:
Mus musculus, Escherichia coli DNA
Platform:
GPL33919
Series:
GSE275291
Download data: BW
Sample
Accession:
GSM8474252
ID:
308474252

SRA Run Selector

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