GPL6461[Accession] - GEO DataSets

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Items: 5

Select item 2000105491.

Identification of Vaccinia Virus HLA Class I Peptides Presented by the Immune System

(Submitter supplied) Seeking to identify HLA class I peptides that originate from vaccinia virus proteins to understand the mechanism of immune protection. Note that vaccinia-infected B cells will still continue to present (primarily) a wide variety of peptides originating from endogenous proteins; this data set contains evidence for more than 5000 such peptides. The objective and challenge is to detect and identify the peptides that originate from the pathogen (vaccinia virus) in the presence (background) of this large number of endogensous 'self' peptides. more...

Organism:: Bos taurus; Homo sapiens; Vaccinia virus

Type:: Protein profiling by Mass Spec

Platform:: GPL6461
3 Samples

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Accession:: GSE10549

ID:: 200010549

Select item 1000064612.

2D-LC MS/MS Analyses of HLA Class I Peptides Isolated from B-cells Infected with Vaccinia Virus

(Submitter supplied) B-cell cultures were infected with live vaccinia virus (NYBOH strain). Cells were lysed, HLA/peptide complexes were enriched by immunoaffinity (W6/32 mAb), peptides were released with acid and separated from HLA proteins by 10 kDa ultrafilitration. HLA class I peptides were separated and identified by 2-dimensional liquid chromatography and tandem mass spectrometry to identify class I peptides derived from vaccinia proteins, that were being presented to the immune system.