GEO DataSets

(Submitter supplied) 9G4+ IgG antibodies expand in SLE in a disease specific fashion and react with different lupus antigens including B cell antigens and apoptotic cells. Their shared use of VH4-34 represents a unique system to understand the molecular basis of lupus autoreactivity. Understanding the participation of apoptotic cells, a rich source of self-antigens including chromatin, in the diversification and selection of autoreactive memory B cells is particularly important in SLE where these cells accumulate in the germinal centers and may activate pathogenic autoreactive B cells. more...

Organism:

Homo sapiens; Mus musculus; Rattus norvegicus

Type:

Protein profiling by protein array

Platform:

GPL17684

3 Samples

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(Submitter supplied) Dilute peptides, proteins, protein complexes, or other antigens in PBS-0.1% Tween20 to 0.1 - 0.2 mg/mL. Transfer diluted antigens transferred to 384-well print plates (15 μL/well). Antibody or other proteins/peptides prelabeled with Cy-3 and / or Cy-5 (diluted to 0.1 μg/μL) can be added to the print plate to serve as marker spots to orient the arrays post-scanning. Using a biorobotic arrayer (MicroGrid 610) and quill pins (Genomic Solution) to print antigens on nitrocellulose coated microarray slides (FAST Slide, 16 pad, Whatman Inc). more...

Organism:

Homo sapiens; Mus musculus; Rattus norvegicus

1 Series

3 Samples

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