GSM126510[Accession] - GEO DataSets

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Items: 3

Select item 2000054921.

Gene expression in the duodenum of sheep genetically resistant or susceptible to nematodes

(Submitter supplied) This experiment compared gene expression in the duodenum of [1] weaned genetically resistant sheep and weaned genetically susceptible sheep (84 days old) [2] genetically resistant sheep and genetically susceptible sheep that have been naturally challenged once with nematodes (175 days old) and [3] genetically resistant sheep and genetically susceptible sheep that have been naturally challenged twice with nematodes (276 days old). more...

Organism:: Ovis aries

Type:: Expression profiling by array

Platforms:: GPL4077 GPL4076 GPL4072
48 Samples

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Accession:: GSE5492

ID:: 200005492

Select item 1000040772.

20K Ovita Ovine Array print 73

(Submitter supplied) This array contains spotted PCR products from an ovine EST collection. Included are housekeeping genes, water blanks (negative PCR) and no DNA blanks (to estimate carryover) Protocol: Ovine EST inserts were amplified in 96 well plates using the universal primers SP6 and T7 in 50 ul reactions (1.5 mM MgCl2, 2 mM each dNTP, 45 pmol primers and 2.5 units AB Red Hot Taq polymerase). The reactions were denatured at 94C for 3 minutes then cycled 36 times at 94C for 45 sec, 55C for 45 sec and 72C for 60 sec. more...