GSM1600649[Accession] - GEO DataSets

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Items: 4

Select item 2000655971.

Genome-wide identification and analysis of mRNA and miRNA expression in MEFs, ESCs, and iPSCs

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by array; Non-coding RNA profiling by array

Platforms:: GPL15216 GPL6887
24 Samples

Download data: IDAT, TXT

Series

Accession:: GSE65597

ID:: 200065597

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000655962.

Genome-wide identification and analysis of microRNA expression in MEFs, ESCs, and iPSCs

(Submitter supplied) Microarray analysis of miRNA—Two micrograms of total RNA was extended at the 3′ terminus with a poly(A) tail using poly(A) polymerase. An oligonucleotide tag was then ligated to the poly(A) tail for later fluorescent dye staining. Hybridization was performed overnight on a µParaflo microfluidic chip using a microcirculation pump (Atactic Technologies). On the microfluidic chip, each detection probe consisted of a chemically modified nucleotide coding segment complementary to the target microRNA (miRBase, http://mirbase.org) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. more...

Organism:: Mus musculus

Type:: Non-coding RNA profiling by array

Platform:: GPL15216
12 Samples

Download data: TXT

Series

Accession:: GSE65596

ID:: 200065596

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000152163.

LC_MRA-1002_miRMouse_18 (miRNA ID version)

(Submitter supplied) Number of unique mature miRNA sequences: 1141 Number of repeats: 3 Number of unique controls: 49 Based on Sanger miRBase release 18. Protocol: Arrays are made using µParaflo Microfluidic Chip Technology.