GEO DataSets

(Submitter supplied) DNA methyltransferase 3A (DNMT3A) is frequently mutated in hematological cancers; however, the underlying oncogenic mechanism remains elusive. Here, we report that DNMT3A mutational hotspot at Arg882 (DNMT3A R882H) cooperates with NRAS mutation to transform hematopoietic stem/progenitor cells and induce acute leukemia development. Mechanistically, DNMT3A R882H directly binds to and potentiates transactivation of stemness genes critical for leukemogenicity including Meis1, Mn1 and Hoxa gene cluster. more...

Organism:

Mus musculus

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platforms:

GPL1261 GPL13112 GPL17400

46 Samples

Download data: BEDGRAPH, BIGBED, CEL

(Submitter supplied) DNA Methyltransferase 3A (DNMT3A) is frequently mutated in various hematopoietic malignancies; however, the underlying oncogenic mechanisms remain elusive. Here, we report that DNMT3A mutational ‘hotspot’ at Arg882 (i.e., DNMT3A-R882H) cooperates with constitutively activated RAS in transforming murine hematopoietic stem/progenitor cells (HSPCs) ex vivo and inducing acute leukemias in vivo. DNMT3A-R882H potentiates aberrant transactivation of ‘stemness’ gene expression programs, notably transcription factors Meis1, Hox-A, Mn1 and Mycn. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17400

12 Samples

Download data: CEL

(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html MoGene-2_1-st-v1.na33.2.mm10.transcript.csv #%create_date=Thu Mar 28 17:33:46 2013 PDT #%chip_type=MoGene-2_1-st-v1 #%lib_set_name=MoGene-2_1-st #%lib_set_version=v1 #%genome-species=Mus musculus #%genome-version=mm10 #%genome-version-ucsc=mm10 #%genome-version-ncbi=38 #%genome-version-create_date=2011-12-00 #%genome-lifted-method=liftOver #%genome-lifted_from-species=Mus musculus #%genome-lifted_from-version-ucsc=mm10 #%genome-lifted_from-version-ncbi=38 #%netaffx-annotation-date=2012-09-30 #%netaffx-annotation-netaffx-build=33 Protocol: see manufacturer's web site

Organism:

Mus musculus

214 Series

6 Related Platforms

3551 Samples

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