GSM1986930[Accession] - GEO DataSets

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Items: 4

Select item 2001031121.

GC-rich coding sequences reduce transposon-like sRNA-mediated transgene silencing in Arabidopsis and maize

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Zea mays; Arabidopsis thaliana

Type:: Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:: GPL15463 GPL13222 GPL20156
76 Samples

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Accession:: GSE103112

ID:: 200103112

Select item 2000764062.

GC-rich coding sequences reduce transposon-like sRNA-mediated transgene silencing in Arabidopsis and maize

(Submitter supplied) The molecular basis of transgene susceptibility to silencing is poorly characterized in plants, thus we evaluated several transgene design parameters as means to reduce heritable sRNA-mediated transgene silencing. Analyses of Arabidopsis plants with transgenes encoding a microalgal polyunsaturated fatty acid (PUFA) synthase revealed that small RNA (sRNA)-mediated silencing, combined with the use of repetitive regulatory elements, led to aggressive transposable element (TE)-like silencing of canola-biased PUFA transgenes. more...

Organism:: Arabidopsis thaliana

Type:: Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing