GSM306146[Accession] - GEO DataSets

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Items: 3

Select item 2000137581.

C4 Photosynthesis: Gene expression in mesophyll and bundle sheath cells of bundle sheath defective2 mutant

(Submitter supplied) Zea mays is a C4 plant that utilizes two distinct cell types, mesophyll (M) and bundle sheath (BS), to cooperatively fix carbon. Regulation of M and BS cell differentiation is poorly understood. Here, we explore the transcriptional networks of M and BS cells by microarray analysis. The maize mutant bundle sheath defective2 lacks the accumulation of Rubisco small and large subunits (Roth et al 1996; Brutnell et al 1999) and cannot perform the Calvin Cycle (Smith et al 1998). more...

Organism:: Zea mays

Type:: Expression profiling by array

Platforms:: GPL5439 GPL5440
24 Samples

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Accession:: GSE13758

ID:: 200013758

Analyze with GEO2R

Select item 1000054392.

Maize oligo array version 1.9 array A

(Submitter supplied) Spotted oligo array on glass. The whole maize array set 1.9 version contains 56310 unique probes from maize and array A contains 29270 of them. The number of total spots including empty and controls is 32448. Protocol: REHYDRATION AND PRINTING OF THE OLIGONUCLEOTIDE MICROARRAYS 1.Add 15 uL of 3XSSC to each well of the 300 pM oligonucleotide set to make a 20 μM oligonucleotide solution. 2.Transfer onto an orbital shaker for 1 h at RT at 100 rpm. more...