(Submitter supplied) We report the application of single-molecule-based sequencing technology for high-throughput profiling of genes regulated under EWSR1-FLI1 expression in A673/TR/shEF. EWSR1-FLI1 specific small hairpin RNA was induced in A673/TR/shEF cells by adding DOX at 1 ug/mL. After 7 days, DOX was removed to allow silencing of the shRNA and induction of EWSR1-FLI1. Cells were harvested at seven different times points: 0 day (d7), 2 days (d9), 3 days (d10), 4 days (d11), 7 days (d14), 10 days (d17) and 15 days (d22) after DOX removal.
- Organism:
- Homo sapiens
- Type:
- Expression profiling by high throughput sequencing
- Platform:
- GPL16791
- 598 Samples
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